From Brindisi Airport:

On leaving the airport follow signs for Bari. This will eventually bring you onto the SS379 dual carriageway. Leave this at the Ostuni/Villanova junction (there will be earlier turnoffs for Ostuni... don’t be tempted to take them). From that junction follow signs for Ostuni. After about 10 mins and going straight across 4 roundabouts you come to a t-junction on the outskirts of Ostuni, opposite the t-junction is a garage (ENI). Turn left here and follow the road up the hill (100m) and bear right following a sign for Altre Direzioni. Avoid signs indicating Centro. Keep following Altre Direzioni signs until you pick up signs for Ceglie Messapica. Follow these until you are on a long straight road that leaves Ostuni. After about 6km (4 miles) look for a sign on your left for Casina Vitale soon on your right there is a sign ‘Birinbau’ about a further half km on the right is a red triangle sign showing 2 roads to the right and one to the left. Drive slowly now. The road to the left is the one you want. 300m after this sign there is a sign on the right for Masseria Fragnite/ Antimo’s /Macelleria  Aziendale. Immediately turn left at this sign. Then take the first right (about 300m): follow signs for Antimo’s /Macelleria  Aziendale (at night only the yellow sign for Antimo’s shows up). After about 1km you come to a T-junction, turn left here. Stay on this road (do not take the left turn). After 1km the road makes a sharp right and goes up a hill, you will see a long white wall on your left with a black wrought iron gate. Enter here and you are at Villa Marianna.

From Bari Airport:

On leaving the airport follow signs for Bari. keep following Bari signs until you see Brindisi signs. Follow Brindisi signs and this will eventually bring you onto SS16. Stay on SS16 (which become SS379 at Fasano) until you see Ostuni/Villanova signs (about 1 hr from Bari). Leave SS379 here, don’t be tempted by earlier Ostuni signs. Now follow directions as in Brindisi Airport above.